This laboratory test is performed on an ascitic fluid sample to detect the presence and identify bacteria and fungi. This test helps the doctor in classifying bacteria grown in culture for starting the appropriate treatment.
Why is this test performed?
This test is performed:
To identify the cause of bacterial or sometimes fungal infection
To identify the shape and class of bacteria i.e. gram positive or gram- negative.
The patient is asked to lay on a bed tilted at 45-60 degrees
Paracentesis: In this process, a little area on your abdomen is cleaned and numbed to remove ascitic fluid from the peritoneal cavity with a needle.
The sample is collected in a container and sent to the laboratory for analysis.
What is Paracentesis?
Paracentesis is a procedure to remove ascitic fluid from the abdominal cavity with the help of a needle. It is used for ascitic fluid analysis and removal of excess peritoneal fluid.
Are viruses detected on Gram staining?
No, viruses are not detected on gram staining.
How to prepare for the test?
Your doctor might ask you to empty your bladder before the test. Inform your doctor about your complete medical history especially of pregnancy, allergy, and bleeding problems.
Is the procedure painful?
No, a numbing medicine is used to make it a painless procedure. However, pressure is felt from the needle.
What are the risks of this test?
Damage to vessels and bladder from needle
Low blood pressure.
Positive or negative
Interpretation of test results:
Negative: A negative test result means that either the infection is not caused by bacteria or the bacteria are very less in number to be seen on gram-stain
Positive: A positive test result provides the following details about the bacteria:
Gram negative or positive
Shape and size
Presence of red or white blood cells
Presence of bacteria inside cells.
You should get tested:
If you have sign and symptoms of a bacterial or fungal infection
If your doctor suspects a bacterial or fungal infection