OVERVIEW:
What is the PAS stain lab test?
PAS stain lab test is used to detect the presence of polysaccharide carbohydrates such as glycogen and mucosubstances such as glycoproteins and glycolipids in tissues.
Why is the PAS stain test done?
PAS stain lab test is used for the diagnosis of the following medical conditions:
- Glycogen storage disease,
- Adenocarcinoma,
- Paget disease of the breast,
- Alveolar soft part sarcoma,
- Ewing sarcoma,
- Erythroleukemia.
What does PAS stain test measure?
PAS stain lab test detects the presence of carbohydrates and carbohydrate compounds such as glycogen, mucin, glycolipids, and glycoproteins in tissues.
- Glycogen: it is a polysaccharide of glucose that serves as a form of energy.
- Glycolipid: These are lipids attached with a carbohydrate. They maintain the integrity of cell membrane.
- Glycoprotein: These are proteins attached with carbohydrate and they play a significant role in immunity.
- Mucins: heavily glycosylated proteins. They provide non-immune protection of the oral cavity.
What PAS stain detects?
PAS staining method is used to detect the presence of polysaccharides and mucosubstances in tissues.
Does PAS stain mucin?
The addition of PAS stain to neutral mucins will bring about bright magenta colour.
Which reaction is the basis of assay of PAS?
The PAS stain is a histochemical reaction in which the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin sulfurous acid and form the magenta colour.
What is glycogen?
Glycogen is a multibranched polysaccharide of glucose (a carbohydrate). It is the main storage form of glucose and a source of energy.
What are mucins?
Mucins are large and heavily glycosylated proteins. These are usually found on all the mucosal surfaces. They provide non-immune protection to the oral cavity.
PAS stain lab test is used for the diagnosis of the following medical conditions:
- Glycogen storage disease,
- Adenocarcinoma,
- Paget disease of the breast,
- Alveolar soft part sarcoma,
- Ewing sarcoma,
- Erythroleukemia.